Correct assay design is essential for identifying substrate specificity, steady state characteristics of enzymes and minimizing false positive and negative inhibitors.

Enzyme activity and inhibition assays

We develop enzyme activity assays for protein purification, characterization of enzymes and inhibitor screening. Correct assay design is essential for identifying substrate specificity, steady state characteristics of enzymes, and minimizing false negative and positive inhibitors. Our scientists are expert at enzymatic assays. Assay development at Affina benefits from the availability of correlation with binding assays, interaction with a network of protein purification and characterization labs and our experience in enzymology and screening.
Methods are based on light absorbance, luminescence, FP and HTR-FRET techniques.

  • Design and/or select substrates based on specificity (kcat/Km), sensitivity (signal to noise, detection method, matrix or contaminant effects
  • Design inhibitor screening studies – select substrates, concentrations of substrate and enzyme, buffers, effects of solvents
  • Perform detailed characterization of selected inhibitors – type of inhibition, Ki or IC50 determination, rate of inhibition (slow-binding and irreversible inhibitors), pH dependence

Get Quote

email

phone

203-487-3998

fax

914-418-5278

address

5 Westchester Plaza
Elmsford, NY 10523

© 2014 Affina Biotechnologies, Inc. All rights reserved.